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Dynamic stainless steel high pressure column
Profile：Chromatography column is mainly based on the active ingredients of plants to be separated, chemical intermediates, compounds and other substances and different fillers in the column: First, in different degrees of affinity, Second, in different concentrations of acid and alkali solution Third, in different polar solutions, four, a certain pressure under different flow rates on the filler layered points ...
Detailed description
Product Name: Dynamic stainless steel high-pressure column
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| Product details: | |
Technical Parameters: | |
model | Specifications mm the inside diameter of | Height mm | Volume increase | Pressure resistance mPa | Materials stainless steel | Note |
CX-ZYDT-63 | Φ63 | "1000 | 1 | ≤1.2 | 304/316L | Non-standard design non-standard production Filter type Sintered plate Highly customizable. |
CX-ZYDT-100 | Φ100 | "1000 | 2 | |||
CX-ZYDT-150 | Φ150 | 1500 | 3 | |||
CX-ZYDT-200 | Φ200 | 1500 | 6 | |||
CX-ZYDT-250 | Φ1250 | 1500 | 8.4 | |||
CX-ZYDT-300 | Φ300 | 1500 | 12.5 | |||
CX-ZYDT-400 | Φ400 | 1500 | 16.5 |
The core of the dynamic axial compression column is to control the piston up and down to pack the column, maintain the column pressure and unload the column. It is the best packing technology in the field of preparative chromatography today. The periphery of the piston is equipped with a specially designed seal ring. The seal ring allows the piston to freely slide up and down while maintaining a seal pressure of 1.2 MPa.
Dynamic axial compression technology:
Axial compression means that the compression direction of the piston when preparing the column packing is the longitudinal axis, which is relative to the radial compression and the annular compression. Among them, axial compression has the lowest cost and the highest effect. The following figure is a schematic diagram of several compression methods.
When the user needs to pack a preparative column with an inner diameter of more than 100 mm, the general axial compression technology cannot achieve high separation requirements and only dynamic axial compression technology can be used. Dynamic axial compression technology is the only technology that can maintain good separation performance in all sizes of column chromatography. Piston motion and pressure maintenance are based on a pneumatic (or hydraulic) jack. The pneumatic jack is more flexible than other companies use. Piston technology is easier to operate, safer, and less likely to leak. The following is a schematic diagram of the structure of a dynamic axial compression column:
Dynamic compression means that the preparation column maintains a proper pressure during use, so that the preparation column bed is kept in a compact state, and dynamic compression is relative to non-dynamic compression. Why use dynamic compression technology? The reason is that there are tiny gaps inside the chromatographic bed when the column is packed (the following figure shows the media gaps in the analytical column and the preparative column). The inner diameter of the analytical column is relatively small, and the void is relatively stable due to the support of the nearby column wall; the diameter of the prepared column is relatively large, and the inside of the chromatographic bed becomes unstable due to the distance from the column wall. During use, due to solvent scouring and mechanical vibration, the voids will be destroyed and recombined. Finally, the preparation column bed will be lowered, and a “dead volume” will form at the inlet of the column. If the negative effect of this dead volume is not eliminated in time, then the column efficiency It will fall sharply. In general, when the column inner diameter is greater than 100 mm, mechanical methods must be used to prevent the bed from collapsing or to eliminate the effects of bed collapse.
The structure of the distributor at the inlet of the preparative column and the internal structure of the column has been optimized so that the flow pattern is stable and the chromatographic peaks are not deformed. When the preparative column inlet only has a screen (to prevent the escape of the filler) and no distributor, the flow velocity of the mobile phase is the largest in the middle of the preparation column, and the peripheral flow velocity is slightly slower, thus forming a peak shape, greatly affecting the column. Performance.
The superior performance of dynamic axial compression columns:
Plate number data:
Our company's preparative column uses Dynamic Axial Compression Technology (DAC). The column inlet and outlet and the internal structure of the column have been optimized. The packing can be loaded with the highest efficiency. The number of plates obtained on the prepared column is equivalent to that of the analytical column. The selectivity and resolution of the separation are well preserved from the amplification of the analytical column to the preparation of the column.
Use stability data: Pack a 20 μm silica gel in a 50 mm ID preparative column packed at a pressure of 10 bar and a column height of 30 cm after column packing. In one case, the column bed is at 10 bar dynamics. In the compressed state, the other is the fixed piston position, that is, in the non-dynamic compression state.
The flow rate of the mobile phase was 150 ml/min. The change in the number of theoretical plates of the two columns within 100 h was examined. As shown below.
The number of theoretical plates for a dynamic axially-compressed column can always be maintained at around 40,000, rather than the 10,000 for a dynamic axially-compressed column dropped from 40,000 at the beginning to 100 hours after 100 hours, especially in the first 2 hours. 40000 is reduced to about 20,000, which is almost half.
The non-dynamic axial compression column produces a dead volume during use, so the efficiency of the column decreases, and the generation of dead volume is particularly evident at the beginning of a certain period of time.
Dynamic compression technology can effectively solve this problem, maintain the compression state during the use of the column, never produce "dead volume" problem, always maintain a high number of plates.